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MOBKL3 (aa137-151) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - MOBKL3 (aa137-151) Antibody (internal region) AF3494a
    AF3494a (0.3 µg/ml) staining of Human Frontal Cortex lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IHC - MOBKL3 (aa137-151) Antibody (internal region) AF3494a
    AF3494a (5 µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • WB - MOBKL3 (aa137-151) Antibody (internal region) AF3494a
    AF3494a (0.3µg/ml) staining of Mouse Brain lysate (A) and (1µg/ml) staining of Rat Brain lysate (B) (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q9Y3A3
Other Accession NP_056202.2, NP_955776.1, NP_001094289.1, 25843, 19070 (mouse), 171050 (rat)
Reactivity Human, Mouse, Rat
Predicted Cow, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 26032 Da
Additional Information
Gene ID 25843
Other Names MOB-like protein phocein, 2C4D, Class II mMOB1, Mob1 homolog 3, Mob3, Mps one binder kinase activator-like 3, Preimplantation protein 3, MOB4, MOB3, MOBKL3, PHOCN, PREI3
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMOBKL3 (aa137-151) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MOB4
Synonyms MOB3, MOBKL3, PHOCN, PREI3
Function May play a role in membrane trafficking, specifically in membrane budding reactions.
Cellular Location Cytoplasm, perinuclear region. Membrane; Peripheral membrane protein. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Note=In a perinuclear punctate pattern. Associated with membranes and the Golgi stacks
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize all three reported isoforms (NP_056202.2; NP_955776.1; NP_001094289.1).

References

Threonine 74 of MOB1 is a putative key phosphorylation site by MST2 to form the scaffold to activate nuclear Dbf2-related kinase 1. Hirabayashi S, Nakagawa K, Sumita K, Hidaka S, Kawai T, Ikeda M, Kawata A, Ohno K, Hata Y, Oncogene 2008 Jul 27 (31): 4281-92. PMID: 18362890

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$ 341.00
Cat# AF3494a
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