|Application ||WB, E|
|Other Accession||NP_001119786.1, 619373, 234155 (mouse)|
|Predicted||Human, Pig, Cow|
|Calculated MW||49716 Da|
|Other Names||Ghrelin O-acyltransferase, 2.3.1.-, Membrane-bound O-acyltransferase domain-containing protein 4, O-acyltransferase domain-containing protein 4, MBOAT4, GOAT, OACT4|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mboat4 (mouse) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein|
|Tissue Location||Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues.|
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Provided below are standard protocols that you may find useful for product applications.
Production of n-octanoyl-modified ghrelin in cultured cells requires prohormone processing protease and ghrelin O-acyltransferase, as well as n-octanoic acid. Takahashi T, Ida T, Sato T, Nakashima Y, Nakamura Y, Tsuji A, Kojima M, Journal of biochemistry 2009 Nov 146 (5): 675-82. PMID: 19628676
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