Mboat4 (mouse) Antibody (internal region)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q96T53 |
Other Accession | NP_001119786.1, 619373, 234155 (mouse) |
Reactivity | Mouse |
Predicted | Human, Pig, Cow |
Host | Goat |
Clonality | Polyclonal |
Concentration | 0.5 mg/ml |
Isotype | IgG |
Calculated MW | 49716 Da |
Gene ID | 619373 |
---|---|
Other Names | Ghrelin O-acyltransferase, 2.3.1.-, Membrane-bound O-acyltransferase domain-containing protein 4, O-acyltransferase domain-containing protein 4, MBOAT4, GOAT, OACT4 |
Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Mboat4 (mouse) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MBOAT4 (HGNC:32311) |
---|---|
Synonyms | GOAT, OACT4 |
Function | Catalyzes ghrelin acylation at 'Ser-3' using preferentially octanoyl-CoA, hexanoyl-CoA and decanoyl-CoA as acyl-CoA donors leading to ghrelin activity (PubMed:24045953, PubMed:18443287, PubMed:25562443, PubMed:28134508). In vitro uses also acyl-CoA donors of different lengths from short-chain (C2) to long-chain fatty acids (C16) knowing that acyl-CoA donors from butanoyl-CoA (C4) to dodecanoyl-CoA (C12) are more efficient compared to longer acyl-CoA donors, such as myristoyl- CoA (C14) and palmitoyl-CoA (C16) that are not efficient (PubMed:18443287). |
Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P0C7A3}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P0C7A3} |
Tissue Location | Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
References
Production of n-octanoyl-modified ghrelin in cultured cells requires prohormone processing protease and ghrelin O-acyltransferase, as well as n-octanoic acid. Takahashi T, Ida T, Sato T, Nakashima Y, Nakamura Y, Tsuji A, Kojima M, Journal of biochemistry 2009 Nov 146 (5): 675-82. PMID: 19628676
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.