|Application ||WB, E|
|Other Accession||NP_689639.2, 140564|
|Calculated MW||46598 Da|
|Other Names||DNA dC->dU-editing enzyme APOBEC-3D, A3D, 3.5.4.-, APOBEC3D|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||APOBEC3D (aa61-74) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits antiviral activity against vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination- independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single-or double- stranded RNA. May inhibit the mobility of LTR and non-LTR retrotransposons.|
|Cellular Location||Cytoplasm. Cytoplasm, P-body.|
|Tissue Location||Expressed in lymphoid organs. Also detected in non-lymphoid tissues including lung|
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Provided below are standard protocols that you may find useful for product applications.
APOBEC3B and APOBEC3F inhibit L1 retrotransposition
by a DNA deamination-independent mechanism. Stenglein MD, Harris RS. J Biol Chem. 2006 Jun 23;281(25):16837-41. PMID: 16648136
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