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HSPC117 (aa201-215) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - HSPC117 (aa201-215) Antibody (internal region) AF3554a
    EB10954 (0.3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IHC - HSPC117 (aa201-215) Antibody (internal region) AF3554a
    EB010954 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, Pep-ELISA
Primary Accession Q9Y3I0
Other Accession NP_055121.1, 51493, 28088 (mouse), 362855 (rat)
Reactivity Human
Predicted Mouse, Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 55210 Da
Additional Information
Gene ID 51493
Other Names tRNA-splicing ligase RtcB homolog {ECO:0000255|HAMAP-Rule:MF_03144}, 6.5.1.3 {ECO:0000255|HAMAP-Rule:MF_03144}, RTCB {ECO:0000255|HAMAP-Rule:MF_03144}, C22orf28
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSPC117 (aa201-215) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RTCB {ECO:0000255|HAMAP-Rule:MF_03144}
Synonyms C22orf28
Function Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
Cellular Location Nucleus. Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03144, ECO:0000269|PubMed:24608264} Note=Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low
Research Areas
Citations (0)
citation

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References

HSPC117 is the essential subunit of a human tRNA splicing ligase complex. Popow J, Englert M, Weitzer S, Schleiffer A, Mierzwa B, Mechtler K, Trowitzsch S, Will CL, Lührmann R, Söll D, Martinez J. Science. 2011 Feb 11;331(6018):760-4. PMID: 21311021

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$ 341.00
Cat# AF3554a
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