|Application ||WB, E|
|Other Accession||NP_620708.1, NP_620709.1, 5601, 26420 (mouse), 50658 (rat)|
|Predicted||Rat, Pig, Dog|
|Calculated MW||48139 Da|
|Other Names||Mitogen-activated protein kinase 9, MAP kinase 9, MAPK 9, 188.8.131.52, JNK-55, Stress-activated protein kinase 1a, SAPK1a, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, MAPK9, JNK2, PRKM9, SAPK1A|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MAPK9 / JNK2 beta (aa217-230) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||JNK2, PRKM9, SAPK1A|
|Function||Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692).|
|Cellular Location||Cytoplasm. Nucleus|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize isoforms beta1 and beta 2 (NP_620708.1; NP_620709.1).
Diverse functions of JNK signaling and c-Jun in stress response and apoptosis. Leppä S, Bohmann D. Oncogene. 1999 Nov 1;18(45):6158-62. PMID: 10557107
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