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ABHD14B (aa188-200) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - ABHD14B (aa188-200) Antibody (internal region) AF3761a
    AF3761a (0.3 µg/ml) staining of Human (A), Mouse(B), Rat (C) Liver lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q96IU4
Other Accession NP_116139.1, 84836, 76491 (mouse), 300983 (rat)
Reactivity Human, Mouse, Rat
Predicted Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 22346 Da
Additional Information
Gene ID 84836
Other Names Alpha/beta hydrolase domain-containing protein 14B, Abhydrolase domain-containing protein 14B, 3.-.-.-, CCG1-interacting factor B, ABHD14B, CIB
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsABHD14B (aa188-200) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ABHD14B {ECO:0000303|PubMed:31478652, ECO:0000312|HGNC:HGNC:28235}
Function Acts as an atypical protein-lysine deacetylase in vitro (PubMed:31478652). Catalyzes the deacetylation of lysine residues using CoA as substrate, generating acetyl-CoA and the free amine of protein- lysine residues (PubMed:31478652). Additional experiments are however required to confirm the protein-lysine deacetylase activity in vivo (Probable). Has hydrolase activity towards various surrogate p- nitrophenyl (pNp) substrates, such as pNp-butyrate, pNp-acetate and pNp-octanoate in vitro, with a strong preference for pNp-acetate (PubMed:31478652, PubMed:14672934). May activate transcription (PubMed:14672934).
Cellular Location Cytoplasm. Nucleus. Note=Predominantly cytoplasmic
Tissue Location Ubiquitous (PubMed:14672934). Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney (PubMed:14672934).
Research Areas
Citations (0)
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Background

Reported variants represent identical protein: NP_116139.1, NP_001139786.1. This antibody is expected to NOT cross-react with ABHD14A.

References

Purification, crystallization and preliminary X-ray crystallographic analysis of human CCG1-interacting factor B. Padmanabhan B, Kuzuhara T, Mizuno H, Horikoshi M. Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1479-81. PMID: 11053859

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$ 341.00
Cat# AF3761a
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