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CLO29 (aa30-43) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - CLO29 (aa30-43) Antibody (internal region) AF3778a
    AF3778a (1 µg/ml) staining of HeLa lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8N999
Other Accession NP_001009894.2, 91298
Reactivity Human
Predicted Pig, Sheep, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 37490 Da
Additional Information
Gene ID 91298
Other Names Uncharacterized protein C12orf29, C12orf29
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCLO29 (aa30-43) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RLIG1 (HGNC:25322)
Function Functions as an RNA ligase, in vitro (PubMed:36792600). The ligation reaction entails three nucleotidyl transfer steps (PubMed:36792600). In the first step, the RNA ligase reacts with ATP in the absence of nucleic acid to form a covalent ligase-AMP intermediate and release pyrophosphate (PubMed:36792600). In step 2, the ligase-AMP binds to the nucleic acid and transfers the adenylate to the 5'-PO4 terminus to form an adenylylated intermediate (PubMed:36792600). In step 3, the RNA ligase directs the attack of the 3'-OH on the 5'- phosphoanhydride linkage, resulting in a repaired 3'-5' phosphodiester and release of AMP (PubMed:36792600). Exhibits selectivity for single- stranded RNA substrates and may not have nick-sealing activity on double-stranded DNA-RNA hybrids (PubMed:36792600). May play a role in maintaining RNA integrity under stress conditions, for example in response to reactive oxygen species (ROS) (PubMed:36792600).
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize isoform 1 (Q8N999-1) and isoform 3 (Q8N999-3) as descibed in UniprotKB.

References

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$ 341.00
Cat# AF3778a
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Availability: 7-10 days
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