|Application ||WB, E|
|Other Accession||NP_001427.2, 2091, 14113 (mouse), 292747 (rat)|
|Predicted||Mouse, Rat, Pig, Dog, Cow|
|Calculated MW||33784 Da|
|Other Names||rRNA 2'-O-methyltransferase fibrillarin, 2.1.1.-, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, FBL, FIB1, FLRN|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Fibrillarin / FBL Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).|
|Cellular Location||Nucleus, nucleolus Note=Fibrillar region of the nucleolus|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Association of guanine nucleotide-exchange protein BIG1 in HepG2 cell nuclei with nucleolin, U3 snoRNA, and fibrillarin. Padilla PI, Uhart M, Pacheco-Rodriguez G, Peculis BA, Moss J, Vaughan M. Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3357-61. Epub 2008 Feb 21. PMID: 18292223
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