|Application ||WB, E|
|Other Accession||NP_001427.2, 2091, 14113 (mouse), 292747 (rat)|
|Predicted||Mouse, Rat, Pig, Dog, Cow|
|Calculated MW||33784 Da|
|Other Names||rRNA 2'-O-methyltransferase fibrillarin, 2.1.1.-, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, FBL, FIB1, FLRN|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||fibrillarin / FBL (aa99-110) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).|
|Cellular Location||Nucleus, nucleolus Note=Fibrillar region of the nucleolus|
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Provided below are standard protocols that you may find useful for product applications.
Association of guanine nucleotide-exchange protein BIG1 in HepG2 cell nuclei with nucleolin, U3 snoRNA, and fibrillarin. Padilla PI, Uhart M, Pacheco-Rodriguez G, Peculis BA, Moss J, Vaughan M. Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3357-61. Epub 2008 Feb 21. PMID: 18292223
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