|Other Accession||NP_002408.3, NP_001139438.1, 4288|
|Calculated MW||358694 Da|
|Other Names||Antigen KI-67, MKI67|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Antigen KI-67 / MKI67 (aa98-110) Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).|
|Cellular Location||Chromosome. Nucleus. Nucleus, nucleolus Note=Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106)|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize both reported isoforms (NP_002408.3; NP_001139438.1).
Claudins and ki-67: potential markers to differentiate low- and high-grade transitional cell carcinomas of the urinary bladder. Törzsök P, Riesz P, Kenessey I, Székely E, Somorácz A, Nyirády P, Romics I, Schaff Z, Lotz G, Kiss A. J Histochem Cytochem. 2011 Nov;59(11):1022-30. PMID: 22043024
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