|Application ||WB, E|
|Other Accession||NP_006488.2, NP_001091672.1, 3090, 15248 (mouse), 303310 (rat)|
|Predicted||Mouse, Rat, Pig, Dog, Cow|
|Calculated MW||76508 Da|
|Other Names||Hypermethylated in cancer 1 protein, Hic-1, Zinc finger and BTB domain-containing protein 29, HIC1, ZBTB29|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HIC1 (aa396-410) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. The regulation of SIRT1 transcription in response to nutrient deprivation seems to involve CTBP1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF- responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription from ACKR3, FGFBP1 and EFNA1.|
|Tissue Location||Ubiquitously expressed with highest levels found in lung, colon, prostate, thymus, testis and ovary Expression is absent or decreased in many tumor cells|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize both reported isoforms (NP_006488.2; NP_001091672.1).
Identification of novel subregions of LOH in gastric cancer and analysis of the HIC1 and TOB1 tumor suppressor genes in these subregions. Yu J, Liu P, Cui X, Sui Y, Ji G, Guan R, Sun D, Ji W, Liu F, Liu A, Zhao Y, Yu Y, Jin Y, Bai J, Geng J, Xue Y, Qi J, Lee KY, Fu S. Mol Cells. 2011 Jul;32(1):47-55. PMID: 21533545
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