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NCKAP1 Antibody (internal region)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| E |
|---|---|
| Primary Accession | Q9Y2A7 |
| Other Accession | NP_038464.1, NP_995314.1, 10787, 50884 (mouse), 58823 (rat) |
| Predicted | Human, Mouse, Rat, Pig, Dog, Cow |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 0.5 mg/ml |
| Isotype | IgG |
| Calculated MW | 128790 Da |
| Gene ID | 10787 |
|---|---|
| Other Names | Nck-associated protein 1, NAP 1, Membrane-associated protein HEM-2, p125Nap1, NCKAP1, HEM2, KIAA0587, NAP1 |
| Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NCKAP1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NCKAP1 |
|---|---|
| Synonyms | HEM2, KIAA0587, NAP1 |
| Function | Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes. |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P28660}; Single-pass membrane protein {ECO:0000250|UniProtKB:P28660}; Cytoplasmic side {ECO:0000250|UniProtKB:P28660}. Cell projection, lamellipodium membrane {ECO:0000250|UniProtKB:P28660}; Single-pass membrane protein {ECO:0000250|UniProtKB:P28660}; Cytoplasmic side {ECO:0000250|UniProtKB:P28660}. Note=At the interface between the lamellipodial actin meshwork and the membrane {ECO:0000250|UniProtKB:P28660} |
| Tissue Location | Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle. Expressed in cells of various brain regions including Purkinje cells and dentate nucleus of the cerebellum, CA4 region and dentate gyrus of the hippocampus, and in frontal gray and white matter (PubMed:28940097). |
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Provided below are standard protocols that you may find useful for product applications.
Background
This antibody is expected to recognize both reported isoforms (NP_038464.1; NP_995314.1).
References
Molecular cloning of a novel apoptosis-related gene, human Nap1 (NCKAP1), and its possible relation to Alzheimer disease. Suzuki T, Nishiyama K, Yamamoto A, Inazawa J, Iwaki T, Yamada T, Kanazawa I, Sakaki Y. Genomics. 2000 Jan 15;63(2):246-54. PMID: 10673335
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