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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Goat Antibodies   >   betaCstF-64 variant 1/ betaCstF-64 variant 3 (mouse) Antibody (internal region)   

betaCstF-64 variant 1/ betaCstF-64 variant 3 (mouse) Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - betaCstF-64 variant 1/ betaCstF-64 variant 3 (mouse) Antibody (internal region) AF3891a
    HeLa lysate either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing Mouse ßCstf-64 (DYKDDDDK tagged) immunoprecipitated using AF3891a (2ug) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDDDDK (1/1000). Mock-transfected HeLa in Lane 1. A 1/40th part of the lysate was loaded in lanes 2 and 3 before IP and in lanes 4 an 5 after IP. Primary incubations were for 1 hour. Detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.
  • WB - betaCstF-64 variant 1/ betaCstF-64 variant 3 (mouse) Antibody (internal region) AF3891a
    AF3891a (2ug) used to pull down ßCstf-64 from Mouse Brain (lane 5) lysate (500ug protein) while it cannot pull down Cstf-64 from Mouse Liver (lane 6) lysate (500ug) protein) using Protein G-coated magnetic beads. A 1/40th part of the lysates were loaded in lanes 1 (Brain) and 2 (Liver) before IP and in lanes 3 (Brain) and 4 (Liver) after IP. Western blot probed with mouse anti-ßCstf-64/Cstf-64 antibody and detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8BIQ5.2
Other Accession ACF05702.1, ACF05700.1, 108062 (mouse)
Reactivity Mouse
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Additional Information
Other Names Cstf2; cleavage stimulation factor, 3' pre-RNA subunit 2; 64kDa; C630034J23Rik; Cstf64; CF-1 64 kDa subunit; CSTF 64 kDa subunit; alphaCstF-64 variant 4; betaCstF-64 variant 1; betaCstF-64 variant 3; cleavage stimulation factor 64 kDa subunit; cleavage st
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsbetaCstF-64 variant 1/ betaCstF-64 variant 3 (mouse) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
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This antibody recognizes mouse variants 1 and 3 of the reported beta isoform (ACF05702.1; ACF05700.1).


A family of splice variants of CstF-64 expressed in vertebrate nervous systems. Shankarling GS, Coates PW, Dass B, Macdonald CC. BMC Mol Biol. 2009 Mar 12;10:22. PMID: 19284619

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$ 335.00
Cat# AF3891a
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