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ERK3 / MAPK6 Antibody (internal region, near C-Term)

Peptide-affinity purified goat antibody

  • WB - ERK3 / MAPK6 Antibody (internal region, near C-Term) AF3922a
    HEK293 lysate (10ug protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with AF3922a (0.5ug/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.
  • IP - ERK3 / MAPK6 Antibody (internal region, near C-Term) AF3922a
    AF3922a (1.5ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16659
Other Accession NP_002739.1, 5597, 50772 (mouse), 58840 (rat)
Reactivity Mouse
Predicted Human, Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 82681 Da
Additional Information
Gene ID 5597
Other Names Mitogen-activated protein kinase 6, MAP kinase 6, MAPK 6,, Extracellular signal-regulated kinase 3, ERK-3, MAP kinase isoform p97, p97-MAPK, MAPK6, ERK3, PRKM6
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsERK3 / MAPK6 Antibody (internal region, near C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAPK6
Synonyms ERK3, PRKM6
Function Atypical MAPK protein. Phosphorylates microtubule- associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).
Cellular Location Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following interaction with MAPKAPK5.
Tissue Location Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts
Research Areas
Citations (0)

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Reported variants represent identical protein (NP_056621.4; NP_081694.1).


The extracellular signal-regulated kinase 3 (mitogen-activated protein kinase 6 [MAPK6])-MAPK-activated protein kinase 5 signaling complex regulates septin function and dendrite morphology. Brand F, Schumacher S, Kant S, Menon MB, Simon R, Turgeon B, Britsch S,
Meloche S, Gaestel M, Kotlyarov A. Mol Cell Biol. 2012 Jul;32(13):2467-78. PMID: 22508986

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$ 325.00
Cat# AF3922a
(40 western blots)
Availability: 7-10 days
Bulk Size
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