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CBFB Antibody (internal region, near C-Term)

Peptide-affinity purified goat antibody

     
  • WB - CBFB Antibody (internal region, near C-Term) AF4001a
    AF4001a (0.3 µg/ml) staining of Daudi lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q13951
Other Accession NP_074036.1, NP_001746.1, 865, 12400 (mouse)
Reactivity Human
Predicted Mouse, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 21508 Da
Additional Information
Gene ID 865
Other Names Core-binding factor subunit beta, CBF-beta, Polyomavirus enhancer-binding protein 2 beta subunit, PEA2-beta, PEBP2-beta, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit, CBFB
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCBFB Antibody (internal region, near C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CBFB
Function CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM- CSF promoters. CBFB enhances DNA binding by RUNX1.
Cellular Location Nucleus.
Research Areas
Citations (0)

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Background

This antibody is expected to recognize reported isoform 1 (NP_074036.1) only, however it is expected to recognize reported mouse isoforms 1 (NP_071704.3), 2 (NP_001154928.1) and 3 (NP_001154929.1).

References

Vif proteins of human and simian immunodeficiency viruses require cellular CBF? to degrade APOBEC3 restriction factors. Hultquist JF, Binka M, LaRue RS, Simon V, Harris RS. Journal of virology 2012 Mar 86 (5): 2874-7. PMID: 22205746

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$ 335.00
Cat# AF4001a
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