|Application ||WB, E|
|Other Accession||NP_000005.2, 2|
|Calculated MW||163291 Da|
|Other Names||Alpha-2-macroglobulin, Alpha-2-M, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, A2M, CPAMD5|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||alpha-2-macroglobulin Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.|
|Tissue Location||Secreted in plasma.|
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Provided below are standard protocols that you may find useful for product applications.
Inhibition of beta2-microglobulin amyloid fibril formation by alpha2-macroglobulin. Ozawa D, Hasegawa K, Lee YH, Sakurai K, Yanagi K, Ookoshi T, Goto Y, Naiki H. The Journal of biological chemistry 2011 Mar 286 (11): 9668-76. PMID: 21216953
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