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Tropomyosin 4 / TPM4 Antibody (C-Term)

Peptide-affinity purified goat antibody

     
  • WB - Tropomyosin 4 / TPM4 Antibody (C-Term) AF4101a
    AF4101a (0.03 µg/ml) staining of Human Platelets lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • WB - Tropomyosin 4 / TPM4 Antibody (C-Term) AF4101a
    AF4101a (0.1 µg/ml) staining of NIH3T3 lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • WB - Tropomyosin 4 / TPM4 Antibody (C-Term) AF4101a
    AF4101a (0.5 µg/ml) staining of Pig Heart lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P67936
Other Accession NP_001138632.1, NP_003281.1, 7171, 326618 (mouse), 24852 (rat)
Reactivity Human, Mouse
Predicted Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 28522 Da
Additional Information
Gene ID 7171
Other Names Tropomyosin alpha-4 chain, TM30p1, Tropomyosin-4, TPM4
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTropomyosin 4 / TPM4 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TPM4
Function Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Detected in cardiac tissue and platelets, the form found in cardiac tissue is a higher molecular weight than the form found in platelets. Expressed at higher levels in the platelets of hypertensive patients with cardiac hypertrophy than in the platelets of hypertensive patients without cardiac hypertrophy (at protein level).
Research Areas
Citations (0)

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Background

This antibody is expected to recognize both reported isoforms (NP_001138632.1; NP_003281.1).

References

Tropomyosin 4 defines novel filaments in skeletal muscle associated with muscle remodelling/regeneration in normal and diseased muscle. Vlahovich N, Schevzov G, Nair-Shaliker V, Ilkovski B, Artap ST, Joya JE, Kee AJ, North KN, Gunning PW, Hardeman EC. Cell motility and the cytoskeleton 2008 Jan 65 (1): 73-85. PMID: 17968984

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$ 335.00
Cat# AF4101a
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