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CAPG Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - CAPG Antibody (internal region) AF4103a
    AF4103a (0.3 µg/ml) staining of U937 (A) and Human Spleen (B) lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • WB - CAPG Antibody (internal region) AF4103a
    AF4103a (0.3 µg/ml) staining of Periheral Blood Lymphocytes (AF4103a lane A), AF4104a lane B) lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P40121
Other Accession NP_001738.2, NP_001243069.1, 822
Reactivity Human
Predicted Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 38499 Da
Additional Information
Gene ID 822
Other Names Macrophage-capping protein, Actin regulatory protein CAP-G, CAPG, AFCP, MCP
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCAPG Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CAPG
Synonyms AFCP, MCP
Function Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
Cellular Location Nucleus. Cytoplasm Melanosome. Cell projection, lamellipodium {ECO:0000250|UniProtKB:P24452}. Cell projection, ruffle {ECO:0000250|UniProtKB:P24452}. Note=In macrophages, may be predominantly cytoplasmic. Nuclear localization was observed in fibroblasts. In macrophages, present at the membrane-cytoplasm interface. In activated macrophages, concentrated in the ruffles of the leading lamellipodia. {ECO:0000250|UniProtKB:P24452}
Tissue Location Macrophages and macrophage-like cells.
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize both reported isoforms (NP_001738.2; NP_001243069.1). Reported variants represent identical protein: NP_001243068.1, NP_001738.2.

References

The actin-capping protein CapG localizes to microtubule-dependent organelles during the cell cycle. Hubert T, Van Impe K, Vandekerckhove J, Gettemans J. Biochemical and biophysical research communications 2009 Feb 380 (1): 166-70. PMID: 19166812

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$ 341.00
Cat# AF4103a
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Availability: 7-10 days
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