|Application ||WB, E|
|Other Accession||NP_005165.1, NP_001001973.1, 509, 11949 (mouse), 116550 (rat)|
|Predicted||Mouse, Dog, Cow|
|Calculated MW||32996 Da|
|Other Names||ATP synthase subunit gamma, mitochondrial, F-ATPase gamma subunit, ATP5C1, ATP5C, ATP5CL1|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ATP5C1 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.|
|Cellular Location||Mitochondrion. Mitochondrion inner membrane; Peripheral membrane protein|
|Tissue Location||Isoform Heart is expressed specifically in the heart and skeletal muscle, which require rapid energy supply Isoform Liver is expressed in the brain, liver and kidney. Isoform Heart and Isoform Liver are expressed in the skin, intestine, stomach and aorta|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize both reported isoforms (NP_005165.1; NP_001001973.1).
Mechanically driven ATP synthesis by F1-ATPase. Itoh H, Takahashi A, Adachi K, Noji H, Yasuda R, Yoshida M, Kinosita K. Nature 2004 Jan 427 (6973): 465-8. PMID: 14749837
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.