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VPS16 Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - VPS16 Antibody (internal region) AF4116a
    AF4116a (1 µg/ml) staining of HepG2 lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9H269
Other Accession NP_072097.2, NP_536338.1, 64601, 80743 (mouse), 296159 (rat)
Reactivity Human
Predicted Mouse, Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 94694 Da
Additional Information
Gene ID 64601
Other Names Vacuolar protein sorting-associated protein 16 homolog, hVPS16, VPS16
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVPS16 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VPS16
Function Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:11382755, PubMed:23351085, PubMed:24554770, PubMed:25266290, PubMed:25783203). Required for recruitment of VPS33A to the HOPS complex (PubMed:23901104). Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS33A but not VPS33B (PubMed:25783203). The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG (PubMed:24554770).
Cellular Location Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome. Cytoplasmic vesicle, clathrin- coated vesicle {ECO:0000250|UniProtKB:Q920Q4}. Cytoplasmic vesicle, autophagosome Note=Colocalizes with AP-3, clathrin, Rab5 and Rab7b (By similarity). Cytoplasmic, peripheral membrane protein associated with early endosomes and late endosomes/lysosomes {ECO:0000250|UniProtKB:Q920Q4, ECO:0000305}
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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Background

This antibody is expected to recognize both reported isoforms (NP_072097.2; NP_536338.1).

References

Molecular cloning and characterization of human VPS18, VPS 11, VPS16, and VPS33. Huizing M, Didier A, Walenta J, Anikster Y, Gahl WA, Krämer H. Gene. 2001 Feb 21;264(2):241-7. PMID: 11250079

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$ 335.00
Cat# AF4116a
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