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AMACR Antibody (C-Term)

Peptide-affinity purified goat antibody

     
  • WB - AMACR Antibody (C-Term) AF4124a
    AF4124a (0.5 µg/ml) staining of Human Kidney lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • WB - AMACR Antibody (C-Term) AF4124a
    EB12538 (0.5µg/ml) staining of CaCo-2 (A) and LNCaP (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
    detail
  • WB - AMACR Antibody (C-Term) AF4124a
    EB12538 (0.5µg/ml) staining of Human Kidney (A) and Liver (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, Pep-ELISA
Primary Accession Q9UHK6
Other Accession NP_055139.4, NP_001161067.1, 23600
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 42387 Da
Additional Information
Gene ID 23600
Other Names Alpha-methylacyl-CoA racemase, 5.1.99.4, 2-methylacyl-CoA racemase, AMACR
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAMACR Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AMACR
Function Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed:7649182, PubMed:10655068, PubMed:11060359). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2- (4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed:7649182, PubMed:10655068, PubMed:11060359).
Cellular Location Peroxisome. Mitochondrion
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize isoform 1 (NP_055139.4) and isoform 3 (NP_001161067.1).

References

High-throughput screen identifies novel inhibitors of cancer biomarker a-methylacyl coenzyme A racemase (AMACR/P504S). Wilson BA, Wang H, Nacev BA, Mease RC, Liu JO, Pomper MG, Isaacs WB. Molecular cancer therapeutics 2011 May 10 (5): 825-38. PMID: 21441411

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$ 341.00
Cat# AF4124a
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