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Goat Anti-Vimentin Antibody (C Terminus)

Purified Goat Polyclonal Antibody

     
  • WB - Goat Anti-Vimentin Antibody (C Terminus) AF4203a
    AF4203a (2 µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P08670
Other Accession 22352(mouse), 81818(rat), NP_003371.2
Reactivity Human
Predicted Human, Mouse, Rat, Pig, Cow, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5
Calculated MW 53652 Da
Additional Information
Gene ID 7431
Other Names VIM; vimentin; FLJ36605
Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Immunogen Peptide with sequence C-QVINETSQHHDDLE, from the C Terminus of the protein sequence according to NP_003371.2.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-Vimentin Antibody (C Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152}
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Research Areas
Citations (0)
citation

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References

Diagnostic value of anti-modified citrullinated vimentin in rheumatoid arthritis. Maraina CH, Nurdayana AK, Rusni D, Azwany Y. Int J Rheum Dis. 2010 Oct;13(4):335-9.

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$ 341.00
Cat# AF4203a
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