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Goat Anti-ATG4A Antibody (internal region)

Purified Goat Polyclonal Antibody

  • IHC-P - Goat Anti-ATG4A Antibody (internal region) AF4214a
    AF4214a (2 µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8WYN0
Other Accession NP_443168.2, NP_840054.1
Reactivity Human
Predicted Human
Host Goat
Clonality Polyclonal
Concentration 0.5
Calculated MW 45378 Da
Additional Information
Gene ID 115201
Other Names ATG4A; ATG4 autophagy related 4 homolog A (S. cerevisiae); APG4A; AUTL2; APG4 autophagy 4 homolog A; AUT-like 2, cysteine endopeptidase; OTTHUMP00000062893; autophagin 2; autophagy-related cysteine endopeptidase 2
Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Immunogen Peptide with sequence TEENGTVNDQTFHC, from the internal region of the protein sequence according to NP_443168.2; NP_840054.1.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-ATG4A Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG4A
Synonyms APG4A, AUTL2
Function Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Cellular Location Cytoplasm.
Tissue Location Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.
Research Areas
Citations (0)

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The COOH terminus of GATE-16, an intra-Golgi transport modulator, is cleaved by the human cysteine protease HsApg4A Scherz-Shouval R, Sagiv Y, Shorer H, Elazar Z J Biol Chem. 2003 Apr 18;278(16):14053-8. Epub 2002 Dec 7

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$ 325.00
Cat# AF4214a
(40 western blots)
Availability: 7-10 days
Bulk Size
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