|Application ||IHC, E|
|Other Accession||NP_443168.2, NP_840054.1|
|Calculated MW||45378 Da|
|Other Names||ATG4A; ATG4 autophagy related 4 homolog A (S. cerevisiae); APG4A; AUTL2; APG4 autophagy 4 homolog A; AUT-like 2, cysteine endopeptidase; OTTHUMP00000062893; autophagin 2; autophagy-related cysteine endopeptidase 2|
|Format||Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.|
|Immunogen||Peptide with sequence TEENGTVNDQTFHC, from the internal region of the protein sequence according to NP_443168.2; NP_840054.1.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-ATG4A Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.|
|Tissue Location||Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.|
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Provided below are standard protocols that you may find useful for product applications.
The COOH terminus of GATE-16, an intra-Golgi transport modulator, is cleaved by the human cysteine protease HsApg4A Scherz-Shouval R, Sagiv Y, Shorer H, Elazar Z J Biol Chem. 2003 Apr 18;278(16):14053-8. Epub 2002 Dec 7
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