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Goat Anti-VASP Antibody (internal region)

Purified Goat Polyclonal Antibody

     
  • WB - Goat Anti-VASP Antibody (internal region) AF4219a
    AF4219a (0.1 µg/ml) staining of Human Platelets lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P50552
Other Accession NP_003361.1
Reactivity Human
Predicted Human
Host Goat
Clonality Polyclonal
Concentration 0.5
Calculated MW 39830 Da
Additional Information
Gene ID 7408
Other Names VASP; vasodilator-stimulated phosphoprotein
Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Immunogen Peptide with sequence C-EKTPKDESANQEEP, from the internal region of the protein sequence according to NP_003361.1.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-VASP Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VASP
Function Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell junction, tight junction Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Note=Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. In pre- apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes)
Tissue Location Highly expressed in platelets.
Research Areas
Citations (0)
citation

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References

Vasodilator-stimulated phosphoprotein regulates osteosarcoma cell migration. Wu G, Wei L, Yu A, Zhang M, Qi B, Su K, Hu X, Wang J. Oncology reports 2011 Dec 26 (6): 1609-15.

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$ 341.00
Cat# AF4219a
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Availability: 7-10 days
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