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Anti-14-3-3 sigma / Stratifin Antibody (Internal), Biotinylated

  • WB - Anti-14-3-3 sigma / Stratifin Antibody (Internal), Biotinylated AF4281a
    Biotinylated antibody (0.1 µg/ml) staining of Mouse Skin lysate (35 µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P31947
Other Accession 2810, NP_006133.1, 55948, 313017
Reactivity Human, Mouse
Predicted Human, Mouse, Rat, Pig, Dog
Calculated MW 27774 Da
Additional Information
Gene ID 2810
Other Names cancer; disease; epithelial marker
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAnti-14-3-3 sigma / Stratifin Antibody (Internal), Biotinylated is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SFN
Synonyms HME1
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
Cellular Location Cytoplasm. Nucleus. Secreted. Note=May be secreted by a non-classical secretory pathway
Tissue Location Present mainly in tissues enriched in stratified squamous keratinizing epithelium
Research Areas
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$ 325.00
Cat# AF4281a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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