|Reactivity||Human, Mouse, Rat|
|Calculated MW||46609 Da|
|Homology||Mouse - identical; human -12/13 amino acid residues identical.|
|Other Names||Thyrotropin-releasing hormone receptor, TRH-R, Thyroliberin receptor, Trhr|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CKQKPTEKAANYS, corresponding to amino acid residues 337-349 of rat Thyrotropin-releasing hormone receptor 1 (Accession Q01717). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Thyrotropin-releasing hormone (TRH), is a widely distributed tripeptide which acts as a hormone, a paracrine regulatory factor and a neurotransmitter/neuromodulator; it is expressed in many parts of the brain as well as in various peripheral tissues1,2. In the hypothalamus, TRH is secreted from the paraventricular nucleus (VPN) by TRH neurons prior to a post-translational processing from its precursor, pro-TRH. A key player in the regulation of the hypothalamus-pituitary-thyroid (HPT) axis, TRH is released into the hypophyseal portal circulation of the median eminence and reaches the pituitary, where it binds to TRH receptors, thereby stimulates the secretion of tyrothropin (thyroid-stimulating hormone; TSH). TSH, in turn, affects the thyroid to produce T4 which is subsequently converted into its biologically-active analog, T3 - a negative mediator of TRH. In the tanycytes, ependymal cells of glial origin located in close proximity to the VPN, T4 is converted to T3 by the enzyme deiodinase II (dio2) - a counterpart to deiodinase III (dio3) which conversely inactivates the above mentioned thyroid hormones, thus so indirectly promotes the production of TRHs3,4. TRH receptor 1 (TRH-R1), the only identified TRH receptor in humans, is a G protein-coupled receptor (GPCR) abundant in the anterior pituitary, neuroendocrine system, autonomic nervous system and the visceral regions of the brain2,5. Like all members of GPCRs, TRH receptor has seven transmembrane domains, and extracellular N-terminus and an intracellular C-terminal tail. TRH receptor couples Gq upon activation which in turn leads to the activation of phospholipase C5. From a behavioral aspect, it was shown that TRH-coupled TRH-R1 increases cognitive arousal by directly exciting the sleep/wake system in the lateral hypothalamic area, and inhibits seizure activity in the hippocampus by facilitating GABA release, whereas its deficiency, as observed in mutant model mice, is linked to growth retardation, depression, and anxiety-like behavior1,6,7. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat TRH-R. Anti-Thyrotropin-Releasing Hormone Receptor 1 antibody (#AG1004) can be used in western blot analysis. It has been designed to recognize TRH-R from rat, mouse and human samples.
References 1. Zeng, H. et al. (2007) Mol. Endocrinol. 21, 2795. 2. Sun, Y. et al. (2003) J. Mol. Endocrinol. 30, 87. 3. Costa-e-Sousa, R.H. et al. (2012) Endocrinology 153, 4128. 4. Chiamolera, M.I. and Wondisford, F.E. et al. (2009) Endocrinology 150, 1091. 5. Engel, S. and Gershengorn, M.C. (2007) Pharmacol. Ther. 113, 410. 6. Hara, J. et al. (2009) J. Neurosci. 29, 3705. 7. Deng, P.Y. et al. (2006) J. Physiol. 577, 497.
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