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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   GPCR Antibodies   >   Neurokinin Receptor 1 (NK1) (extracellular) Antibody   

Neurokinin Receptor 1 (NK1) (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - Neurokinin Receptor 1 (NK1) (extracellular) Antibody AG1005-050
    Western blot analysis of rat ovary (lanes 1 and 5), rat brain (lanes 2 and 6), mouse brain membrane (lanes 3 and 7) and SH-SY5Y (lanes 4 and 8) lysate: 1-4. Anti-Neurokinin Receptor 1 (NK1) (extracellular) antibody (#AG1005), (1:200).
    5-8. Anti-Neurokinin Receptor 1 (NK1) (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Neurokinin Receptor 1 (NK1) (extracellular) Antibody AG1005-050
    Expression of Neurokinin Receptor 1 in rat colon Immunohistochemical staining of rat colon paraffin-embedded section using Anti-Neurokinin Receptor 1 (NK1) (extracellular) antibody (#AG1005), (1:100) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. A. NK1 labeling appears in the tubular glands of the mucosa layer. Note that the smooth muscle and lamina propria do not stain. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.
  • FC - Neurokinin Receptor 1 (NK1) (extracellular) Antibody AG1005-050
    Indirect flow cytometry analysis of MEG-O1 living cells:
    — Unstained cells.
    Cells + Anti-Anti-Neurokinin Receptor 1 (NK1) (extracellular) antibody (#AG1005), (5-10 µg/1x106 cells).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14600
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 46366 Da
Homology Mouse- identical; human- 13/15 amino acid residues identical.
Additional Information
Gene ID 24807
Other Names Substance-P receptor, SPR, NK-1 receptor, NK-1R, Tachykinin receptor 1, Tacr1, Tac1r
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CMIEWPEHPNRTYEK, corresponding to amino acid residues 180-194 of rat Neurokinin Receptor 1 (NK1) (Accession P14600). 2nd extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 mg antibody.
Research Areas
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Substance P (SP), Neurokinin A (NKA) and Neurokinin B (NKB) are all peptides belonging to the Tachykinin protein family. These three peptides which demonstrate a quite heterogeneity in their distribution exert their effect via three receptors: Neurokinin 1-3 receptors, members of the G-protein coupled receptor superfamily. However, Neurokinin 1 Receptor (NK1) preferentially binds Substance P, Neurokinin 2 Receptor (NK2) to NKA and Neurokinin 3 Receptor (NK3) to NKB1. Neurokinin receptors are distinguished by their seven transmembrane domains, an extracellular N-terminus and a cytosolic C-terminal. An unusual property of these receptors is the presence of introns as part of their structural organization1-3. Tachykinin receptors undergo alternative splicing. For example, NK1 is detected with different C-terminal lengths. The longer receptor isoform is found in the brain whereas the truncated form is mostly detected in the periphery1,4. Due to the broad expression profile of tachykinin peptides, their respective receptors are also expressed in a similar fashion. NK1 is widely expressed in neurons endothelial cells, muscle and immune system cells. NK2 is broadly expressed in the periphery and its expression in the brain is quite restricted. NK3 on the other hand is largely expressed in the central nervous system and is also detected in the uterus, skeletal muscle, lung and liver1. Neurokinin receptors have been found in many pathophysiological indications and have therefore become targets for the development of pharmacological compounds. Such indications include cancer, psychological disorders, migraine and various inflammations, just to name a few5-8. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Neurokinin Receptor 1. Anti-Neurokinin Receptor 1 (NK1) (extracellular) antibody (#AG1005) can be used in western blot analysis and indirect flow cytometry applications, and has been designed to recognize NK1 from rat, mouse and human samples.


References 1. Pennefather, J.N. et al. (2004) Life Sci. 74, 1445. 2. Hershey, A.D. et al. (1991) J. Biol. Chem. 266, 4366. 3. Gerard, N.P. et al. (1993) Regul. Pept. 43, 21. 4. Caberlotto, L. et al. (2003) Eur. J. Neurosci. 17, 1736. 5. Munoz, M. et al. (2011) Expert Opin. Ther. Targets 15, 889. 6. Ebner, K. et al. (2009) Curr. Pharm. Des. 15, 1647. 7. May, A. and Goadsby, P.J. (2001) Expert Opin. Investig. Drugs 10, 673. 8. Palecek, J. et al. (2003) Neuroscience 116, 565.

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$ 475.00
$ 575.00
Cat# AG1005-050
(40 western blots)
Availability: 2-3 weeks
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