|Reactivity||Human, Mouse, Rat|
|Calculated MW||32445 Da|
|Homology||Mouse, human - identical.|
|Other Names||Trimeric intracellular cation channel type B, TRIC-B, TRICB, Transmembrane protein 38B, Tmem38b|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)GMKEVTRTWKIVG, corresponding to amino acid residues 116-126 of rat TRIC-B (Accession Q68FV1). Intracellular, cytoplasmic loop.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||2 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Intracellular Ca2+ levels are important in proper cellular functions and have prominent roles in various cell signaling pathways and are crucial for muscle contractions. Indeed, an important step leading to muscle contraction is the massive release of Ca2+ ions from the endoplasmic /sarcoplasmic reticulum (ER/SR) to the cytosol. A battery of results suggest that specific K+ channels are important to counteract the Ca2+ outflow in order to neutralize the negative potential created by the movement of Ca2+ ions. It is believed that TRIC channels are responsible for neutralizing this negative potential1,2. Trimeric intracellular cation-specific (TRIC) channels are critical for proper management of intracellular stores. TRIC-A and TRIC-B both belong to this family and are both permeable to monovalent ions with a preference for K+ 3. Both channels are localized to the ER/SR membrane. Each TRIC subunit contains three transmembrane domains, a cytoplasmic C-terminus and a luminal N-terminus. Functional entities are formed by homotrimerizarion4. The activity of TRIC-A is regulated by voltage whereas that of TRIC-B can be regulated by different mechanisms3. Knock out studies of these channels have shown that TRIC-A knock mice are viable while those of TRIC-B die at the neonatal stage5. TRIC-A is mostly expressed in excitable tissues like the brain and muscle while TRIC-B is ubiquitously expressed. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat TRIC-B. Anti-TRIC-B antibody (#AG1007) can be used in western blot analysis, and has been designed to recognize TRIC-B from rat, mouse and human samples.
References 1. Weisleder, N. et al. (2008) Cell Calcium 43, 1. 2. Zhao, X. et al. (2010) J. Biol. Chem. 285, 37370. 3. Silverio, A.L.F. and Saier Jr., M.H. (2011) J. Memb. Biol. 241, 77. 4. Yazawa, M. et al. (2007) Nature 448, 78. 5. Yamazaki, D. et al. (2009) Pharmacol. Ther. 121, 265.
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