|Calculated MW||50336 Da|
|Homology||Mouse - identical. Human- not recommended for use with human samples.|
|Other Names||5-hydroxytryptamine receptor 3B, 5-HT3-B, 5-HT3B, Serotonin receptor 3B, Htr3b|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)HIRQSSAGDFAQIR, corresponding to amino acid residues 213-226 of rat 5-Hydroxytryptamine Receptor 3B (Accession Q9JJ16). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 ml or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.95 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
5-Hydroxytryptamine Receptor 3B (5-HT3B) belongs to the super-family of ligand-gated ion channels. Serotonin receptors, other than 5-HT3 subtype belong to the super-family of G-protein coupled receptors. The 5-HT3 receptor is formed by five subunits arranged around a pore forming unit. Receptors could be either monomeric, such as 5-HT3A or heteromeric entities like 5-HT3A/B. Indeed, the type of channel formed displays different pharmacological and electrophysiological characteristics1,2. To date, five 5-HT3 subunits have been identified 5-HT3A-E, which show variability in the N-terminus and in the transmembrane region2. 5-HT3A and 5-HT3B are the best characterized among the different types. In general, 5-HT3 receptors are located in the peripheral and central nervous system, in lymphocytes and intestinal enterochromaffine cells2. In presynaptic neurons, activation of these receptors leads to an increase in intracellular Ca2+ (by both influx and mobilization of intracellular stores), and modulates the release of a number of neurotransmitters and neuropeptides2. At the postsynaptic level, activation leads to membrane depolarization2,3. The localization of 5-HT3B subunits is somewhat controversial; some studies show that its expression is restricted to the peripheral nervous system and others show that it is also detected in the brain and in hippocampal neurons1,4-7. 5-HT3 receptors have become important targets for which to develop treatments regarding irritable bowel syndrome (IBS), side effects resulting from chemotherapeutic treatment, schizophrenia and bipolar disorder1,2. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat 5-HT3B. Anti-5-Hydroxytryptamine Receptor 3B (extracellular) antibody (#AG1008) can be used in western blot analysis and has been designed to recognize 5-HT3B from rat and mouse samples.
References 1. Thompson, A.J. and Lummis, S.C.R. (2006) Curr. Pharm. Des. 12, 365. 2. Faerber, L. et al. (2007) Eur. J. Pharmacol. 560, 1. 3. Ronde, P. and Nichols, R.A. (1998) J. Neurochem. 70, 1094. 4. Morales, M. et al. (2002) J. Neurosci. 22, 6732. 5. Sudweeks, S.N. et al. (2002) J. Physiol. 544, 715. 6. Davies, P.A. et al. (1999) Nature 397, 359. 7. Monk, S.A. et al. (2001) Neuropharmacology 41, 1023.
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