5-Hydroxytryptamine Receptor 1D (extracellular) Antibody
Affinity purified rabbit polyclonal antibody
|Calculated MW||41540 Da|
|Homology||Mouse - identical; human - 13/14 amino acid residues identical.|
|Other Names||5-hydroxytryptamine receptor 1D, 5-HT-1D, 5-HT1D, Serotonin receptor 1D, Htr1d|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EASNRSLNATGAWD, corresponding to amino acid residues 14-27 of rat 5-hydroxytryptamine receptor 1D (Accession P28565. Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Serotonin (5-hydroxytryptamine, 5-HT) plays unequivocally a most important role in the normal physiology amongst higher eukaryotes, as do most neurotransmitters. The physiological roles of serotonin and its receptors range from appetite, regulation of the circadian rhythm, sexual behavior, memory and nociception. Serotonin is also involved in migraine and some psychiatric diseases such as depression anxiety and aggression1. Serotonin and its receptors also have an influence on the cardiovascular, pulmonary, gastrointestinal (GI) and genitourinary systems2. To date, some fifteen serotonin receptors have been cloned and grouped into seven families (5-HT1-5-HT7) based on their signaling mechanisms. The 5-HT1 receptor class comprises 5 different receptors with ranging homology. Apart from 5-HT3, all serotonin receptors belong to the G-Protein Coupled Receptor superfamily and therefore span the membrane seven times3. Members of 5-HT1 receptor class all couple Gi/0 thereby inhibiting adenylate cyclase. In the central nervous system, 5-HT1D is expressed in the basal ganglia, hippocampus and cortex in rat and in the basal ganglia, midbrain and spinal cord in human. In serotonin neurons, 5-HT1D receptors are autoreceptors thereby inhibiting the release of 5-HT. In GABA, ACh, and glutamate neurons they are heteroreceptors where they control the release of these neurotransmitters4,5. As mentioned above, the serotonin system plays an undeniable role in the perception and processing of pain. Indeed, many migraine sufferers are prescribed triptan-based drugs which activate 5-HT1B and 5-HT1D receptors2. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat 5-Hydroxytryptamine Receptor 1D (5-HT1D). Anti-5-Hydroxytryptamine Receptor 1D (extracellular) antibody (#AG1010) can be used in western blot analysis. It has been designed to recognize 5-Hydroxytryptamine Receptor 1D from human, rat and mouse samples.
1. Sari, Y. (2004) Neurosi. Biobehav. Rev. 28, 565.
2. Berger, M. et al. (2009) Annu. Rev. Med. 60, 355.
4. Filip, M. and Bader, M. (2009) Pharmacol. Rep. 61, 761.
5. Fink, K.B. and Göthert, M. (2007) Pharmacol. Rev. 59, 360.
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