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5-Hydroxytryptamine Receptor 1B (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

     
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  • WB - 5-Hydroxytryptamine Receptor 1B (extracellular) Antibody AG1011-050
    All lanes : Anti-HTR1B Antibody at 1:1000 dilution Lane 1: rat brain lysate Lane 2: rat cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - 5-Hydroxytryptamine Receptor 1B (extracellular) Antibody AG1011-050
    Western blot analysis of lysates from mouse brain, rat brain and human brain tissue lysate(from left to right), using HTR1B Antibody(Cat. #AG1011). AG1011 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - 5-Hydroxytryptamine Receptor 1B (extracellular) Antibody AG1011-050
    Western blot analysis of rat hippocampus lysate: 1. Anti-5-Hydroxytryptamine Receptor 1B (extracellular) antibody (#AG1011), (1:400). 2. Anti-5-Hydroxytryptamine Receptor 1B (extracellular) antibody, preincubated with the control peptide antigen.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P28222
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 43568 Da
Homology Rat - 15/17 amino acid residues identical; mouse - 14/17 amino acid residues identical.
Additional Information
Gene ID 3351
Other Names 5-hydroxytryptamine receptor 1B, 5-HT-1B, 5-HT1B, S12, Serotonin 1D beta receptor, 5-HT-1D-beta, Serotonin receptor 1B, HTR1B, HTR1DB
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CSAKDYIYQDSISLPWK, corresponding to amino acid residues 33-49 of human 5-Hydroxytryptamine Receptor 1B (Accession P28222). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Background

Serotonin (5-hydroxytryptamine, 5-HT) plays unequivocally a most important role in the normal physiology amongst higher eukaryotes, as do most neurotransmitters. The physiological roles of serotonin and its receptors range from appetite, regulation of the circadian rthythm, sexual behavior, memory and nociception. Serotonin is also involved in migraine and some psychiatric diseases such as depression anxiety and aggression1. Serotonin and its receptors also have an influence on the cardiovascular, pulmonary, gastrointestinal (GI) and genitourinary systems2. To date, some fifteen serotonin receptors have been cloned and grouped into seven families (5-HT1-5-HT7) based on their signaling mechanisms. The 5-HT1 receptor class comprises 5 different receptors with ranging homology. Apart from 5-HT3, all serotonin receptors belong to the G-Protein Coupled Receptor superfamily and therefore span the membrane seven times3. The 5-Hydroxytryptamine Receptor 1B (5-HT1B) is involved in several physiological behaviors and psychiatric disease, including locomotor activity, drug abuse reinforcement, migraine, anxiety states and aggressive behaviors1. Pharmacological studies suggest that 5-HT1B is expressed by both serotonergic and non-serotonergic neurons. In the nervous system, this receptor is located in the axon terminal of the plasma membrane of unmyelinated axons. It is predominantly localized on pre-synaptic membranes. Signal transduction studies of the receptor indicate that this receptor is coupled negatively to adenylyl cyclase as its activation induces a decrease in adenylyl cyclase activity1. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of human 5-Hydroxytryptamine Receptor 1B (5-HT1B). Anti-5-Hydroxytryptamine Receptor 1B (extracellular) antibody (#AG1011) can be used for western blot analysis. It has been designed to recognize 5-Hydroxytryptamine Receptor 1B from human, rat and mouse samples.

References

1. Sari, Y. (2004) Neurosi. Biobehav. Rev. 28, 565.
2. Berger, M. et al. (2009) Annu. Rev. Med. 60, 355.
3. http://www.iuphar-db.org/DATABASE/ReceptorFamiliesForward?type=GPCR.

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$ 475.00
$ 575.00
Cat# AG1011-050
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