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5-Hydroxytryptamine Receptor 1A Antibody

Affinity purified rabbit polyclonal antibody

  • WB - 5-Hydroxytryptamine Receptor 1A Antibody AG1012-025
    Western blot analysis of mouse brain membrane (lanes 1 and 3) and rat brain membrane (lanes 2 and 4): 1,2. Anti-5-Hydroxytryptamine Receptor 1A antibody (#AG1012), (1:200). 3,4. Anti-5-Hydroxytryptamine Receptor 1A antibody, preincubated with the control peptide antigen
  • IHC - 5-Hydroxytryptamine Receptor 1A Antibody AG1012-025
    Expression of 5-HT1A in rat cerebellum Immunohistochemical staining of rat cerebellum frozen section using Anti-5-Hydroxytryptamine Receptor 1A antibody (#AG1012), (1:100). 5HT1A is expressed in Purkinje cells (red). Hoechst 33342 is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19327
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 46429 Da
Homology Mouse - identical.
Additional Information
Gene ID 24473
Other Names 5-hydroxytryptamine receptor 1A, 5-HT-1A, 5-HT1A, Serotonin receptor 1A, Htr1a, 5ht1a
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide KKSLNGQPGSGDWRRC, corresponding to amino acid residues 251-266 of rat 5-Hydroxytryptamine Receptor 1A (Accession P19327). 3rd intracellular loop.Will not recognize human 5-Hydroxytryptamine Receptor 1A.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
Citations (0)

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Serotonin [5-hydroxytryptamine (5-HT1)] is an important neurotransmitter in the CNS that exerts its neuronal activity via 5-HT receptors. 5-HT receptors are subdivided into seven families termed 5-HT1 through 5-HT7.1 The 5-HT receptor families belong to the G-Protein Coupled receptor superfamily with the exception of 5-HT3 receptor class which belongs to the ligand-gated ion channels. 2 The 5-HT1 receptor class comprises of five different receptors (5-HT1A, 5-HT1B, 5-HT1D, 5-HT1e AND 5-HT1F) which share 41-63% overall sequence identity and couple preferentially to Gi/Go to inhibit cAMP formation.2 The 5-HT1A receptor is the most widespread of all the 5-HT receptors. In the central nervous system, 5-HT1A receptors exist in the cerebral cortex, hippocampus, septum, amygdala and raphe nucelus in high densities, and was also demonstrated in a variety of tissues from various species.2,3 Activation of 5-HT1A receptor decreases blood pressure and heart rate, involved in learning, memory, relieving anxiety and depression. 1,4 Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat 5-Hydroxytryptamine Receptor 1A (5-HT1A). Anti-5-Hydroxytryptamine Receptor 1A antibody (#AG1012) can be used for western blot and immunohistochemistry applications. It has been designed to recognize 5-Hydroxytryptamine Receptor 1A from rat and mouse samples.


1. Fink, K.B. and Gothert, M. (2007) Pharmacol. Rev. 59, 360.
3. Hoyer, D. et al. (1994) Pharmacol. Rev. 46, 157.
4. Dabire, H. (1991) Therapie 46, 421.

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$ 375.00
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$ 575.00
Cat# AG1012-025
(40 western blots)
Availability: 2-3 weeks
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