Sphingosine 1-Phosphate Receptor 3 (extracellular) Antibody
Affinity purified rabbit polyclonal antibody
|Application ||FC, WB|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||42270 Da|
|Homology||Rat - identical; human - 9/12 amino acid residues identical.|
|Other Names||Sphingosine 1-phosphate receptor 3, S1P receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Lysophospholipid receptor B3, Sphingosine 1-phosphate receptor Edg-3, S1P receptor Edg-3, S1pr3, Edg3, Lpb3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)DYVGKLAGRLRD, corresponding to amino acid residues 23-34 of mouse Sphingosine 1-phosphate receptor 3 (Accession Q9Z0U9). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Sphingosine 1-phosphate (S1P) is an active byproduct of sphingomyelin metabolism. All cells can synthesize this biomolecule but the majority of its synthesis comes from erythrocytes and endothelial cells. In cases of inflammation, mast cells and platelets are the main source of sphingosine 1-phosphate1. S1P cellular functions could be intracellular, where it is synthesized. In addition, upon secretion, it circulates in the blood via its binding to high-density lipoproteins and albumin. When S1P reaches its target cell it could activate 5 different high affinity receptors belonging to the G-protein coupled receptor superfamily: Sphingosine 1-phosphate receptors, termed S1PR1-5 1. Stimulation of S1PR receptors triggers a cascade of signaling events depending on the receptor and on the G-protein it couples. S1PR1 couples to Gi2. S1PR2 can couple to Gs, Gq or G12/13 and S1PR3-5 can couple to Gi or G12/133-4. The pathways activated vary from Ca2+ mobilization, activation or inhibition of adenylate cyclase phospholipase C activation and more. Through the different signaling pathways these receptors activate, S1P1R receptors are implicated in adherens junction assembly, cytoskeletal changes, cell migration, proliferation and apoptosis5. S1PR3 is specifically detected in the brain, heart, spleen, liver, lung, thymus, kidney, testis and skeletal muscle3. S1PR1 and S1PR2 are generally expressed in the CNS, cardiovascular and immune systems1. S1PR4 is specifically expressed in the lymphoid tissue and S1PR5 in natural killer cells and olygodendrocytes1. Malfunction of S1PR receptor signaling is reported in various disorders, for example multiple sclerosis and may be targets for the development of therapeutic drugs1. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse sphingosine 1-phosphate receptor 3. Anti-Sphingosine 1-Phosphate Receptor 3 (extracellular) antibody (#AG1013) can be used in western blot analysis and indirect flow cytometry applications. It has been designed to recognize S1PR3 from human, rat and mouse samples.
1. Hla, T. and Brinkmann, V. (2011) Neurology 76, S3.
2. Rosen, H. et al. (2007) Trends Immunol. 28, 102.
3. Rosen, H. et al. (2009) Annu. Rev. Biochem. 78, 743.
4. Jiang, L.I. et al. (2007) J. Biol. Chem. 282, 10576.
5. Sanchez, T. and Hla, T. (2004) J. Cell. Biochem. 92, 913.
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