|Application ||WB, IHC|
|Calculated MW||38829 Da|
|Homology||Rat - 17/18 amino acid residues identical; human - 16/18 amino acid residues identical.|
|Other Names||Sphingosine 1-phosphate receptor 2, S1P receptor 2, S1P2, Endothelial differentiation G-protein coupled receptor 5, Lysophospholipid receptor B2, Sphingosine 1-phosphate receptor Edg-5, S1P receptor Edg-5, S1pr2, Edg5, Gpcr13, Lpb2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide ERQVALAKVKLYGSDKSC, corresponding to amino acid residues 129-146 of mouse Sphingosine 1-Phosphate Receptor 2 (S1PR2), (Accession P52592). 2nd intracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.95 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (SIP), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2. Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3. Sphingosine 1-Phosphate Receptor 2 (S1PR2) is ubiquitously expressed. S1PR2 mRNA was detected in the brain, heart, spleen, liver, lung, thymus, kidney and skeletal muscle4. Unlike S1PR1, knockout of SIPR2 gene is not lethal5. Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of the mouse Sphingosine 1-Phosphate Receptor 2. Anti-Sphingosine 1-Phosphate Receptor 2 antibody (#AG1014) can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize Sphingosine 1-Phosphate Receptor 2 from mouse, rat and human samples.
1. Pyne, S. and Pyne, N.J. (2000) Biochem. J. 349, 385.
2. Hla, T. et al. (2001) Science 294, 1875.
3. Takuwa, Y. et al. (2002) J. Biochem. 131, 767.
4. Rosen, H. et al (2009) Annu. Rev. Biochem. 78, 743.
5. Rosen, H and Liao, J. (2002) Biochim. Biophys. Acta. 1582, 222.
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