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Sphingosine 1-Phosphate Receptor 1 (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Sphingosine 1-Phosphate Receptor 1 (extracellular) Antibody AG1015-050
    Western blot analysis of RAEC cells (lanes 1 and 3) and in A-10 (lane 2 and 4) cell lysates: 1, 2. Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular) antibody (#AG1015), (1:200). 3, 4. Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Sphingosine 1-Phosphate Receptor 1 (extracellular) Antibody AG1015-050
    Expression of Sphingosine 1-Phosphate Receptor 1 in rat lung Immunohistochemical staining of paraffin embedded rat lung sections using Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular) antibody (#AG1015), (1:100). Staining is present in vascular smooth muscle (black arrows) but not in the muscular layer of bronchi (red arrows). Hematoxilin is used as the counterstain.
  • ICC - Sphingosine 1-Phosphate Receptor 1 (extracellular) Antibody AG1015-050
    Expression of Sphingosine 1-Phosphate Receptor 1 in mouse 3T3 cells Immunocytochemical staining of mouse 3T3 cells using Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular) antibody (#AG1015), (1:50), (red). DAPI is used for nuclear staining (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21453
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 42811 Da
Homology Mouse, rat - 13/14 amino acid residues identical.
Additional Information
Gene ID 1901
Other Names Sphingosine 1-phosphate receptor 1, S1P receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, Sphingosine 1-phosphate receptor Edg-1, S1P receptor Edg-1, CD363, S1PR1, CHEDG1, EDG1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide SDYVNYDIIVRHYN(C), corresponding to amino acid residues 17-30 of human Sphingosine 1-Phosphate Receptor 1 (S1PR1), (Accession P21453). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.75 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (SIP), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2. Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3. Sphingosine 1-Phosphate Receptor 1 (S1PR1) is ubiquitously expressed. S1PR1 mRNA is detected in the brain, heart, spleen, liver, lung, thymus, kidney, skeletal muscle and lymphoid tissues4. Knockout of the gene encoding S1PR1 is lethal and therefore makes in vivo studies difficult 5. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of the human Sphingosine 1-Phosphate Receptor 1. Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular) antibody, (#AG1015) can be used in western blot analysis, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize Sphingosine 1-Phosphate Receptor 1 from human, rat and mouse samples.


1. Pyne, S. and Pyne, N.J. (2000) Biochem. J. 349, 385.
2. Hla, T. et al. (2001) Science 294, 1875.
3. Takuwa, Y. et al. (2002) J. Biochem. 131, 767.
4. Rosen, H. et al. (2009) Annu. Rev. Biochem. 78, 743.
5. Rosen, H and Liao, J. (2002) Biochim. Biophys. Acta. 1582, 222.

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$ 475.00
$ 575.00
Cat# AG1015-050
(40 western blots)
Availability: 2-3 weeks
Bulk Size
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