|Application ||WB, IHC|
|Calculated MW||41200 Da|
|Homology||Mouse - identical; human, pig - 12/15 amino acid residues identical.|
|Other Names||Somatostatin receptor type 2, SS-2-R, SS2-R, SS2R, SRIF-1, Sstr2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)GSNQTEPYYDMTSN, corresponding to amino acid residues 30-43 of rat SSTR2 (Accession number P30680). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Somatostatin is a small cyclic peptide that is widely expressed throughout the central nervous system and peripheral tissues.1 In peripheral tissues, somatostatin exerts inhibitory effects on secretion processes, whereas in the brain, it acts as a neurotransmitter in both a stimulatory and an inhibitory manner.1,2 Somatostatin mediates its action via six high affinity G-protein-coupled receptors (SSTR1, SSTR2a, SSTR2b, SSTR3, SSTR4, and SSTR5), which are encoded by five genes.1,2 Expression of the different receptors is developmentally regulated in a time- and tissue-specific manner.2 Somatostatin receptors have been found on a variety of neuroendocrine tumors, such as paragangliomas, carcinoids, and breast tumors.3 Synthetic peptide derivatives of somatostatin have been successfully used in the treatment of neuroendocrine malignancies and in vivo imaging of tumors that are positive to somatostatin receptors.4 In general, SSTR2 is the most common SSTR subtype found in human tumors, followed by SSTR1, with SSTR3 and SSTR4 being less common. Abgent is pleased to offer a highly specific antibody directed against the N-terminus domain of the rat SSTR2. Anti-Somatostatin Receptor Type 2 (extracellular) antibody (#AG1016), can be used in western blot analysis and immunohistochemical appications, and recognizes SSTR2 in mouse and rat samples.
1. Hofland, L.J. and Lamberts, S.W.J. (2001) Ann. Oncol. 12, S31.
2. Fombonne, J. et al. (2003) Rep. Biol. Endocrinol. 1, 19.
3. Slooter, G.D. et al. (2001) Br. J. Surg. 88, 31.
4. Schulz, S. et al. (2002) Gyn. Oncol. 84, 235.
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