|Reactivity||Human, Mouse, Rat|
|Calculated MW||78888 Da|
|Homology||Mouse, human- identical.|
|Other Names||Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial Na(+) channel subunit alpha, Alpha-ENaC, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, Scnn1a, Renac|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EELDRITEQTLFD, corresponding to amino acid residues 173-185 of rat ENaC־± (Accession P37089). Extracellular loop.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The DEG/ENaC family consists of Na+ selective channels. They are characterized by 2 transmembrane domains with a large extracellular loop, important for channel activity and intracellular N-and C-termini which are important for channel gating and for interacting with regulatory proteins respectively1,2. ENaC is localized to the apical side of polarized epithelial cells like those in the kidney, lung, and distal colon and is responsible for maintaining proper Na+ balance. This channel is formed by oligomerization of three subunits, α, β, and γ usually with the α2β1γ1 combination2,3. In addition, the a subunit which is essential for channel activity undergoes phosphorylation in its C-terminal by SGK1 (serum and glucocorticoid-induced kinase 1) which is known to activate the channel at the plasma membrane4. Pseudohypoaldosteronism type 1 (PHA-1) has an early onset and is depicted by severe dehydration, hyponatremia and hyperkalemia5. One form of the pathology is characterized by mutations in α, β, and γ subunits5. The severity of the diseases depends on the loss of activity of the channel2. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat ENaCα subunit. Anti-ENaCα (extracellular) antibody (#AG1023) can be used in western blot analysis. It has been designed to recognize ENaCα from human, rat and mouse samples.
References 1. Árnadóttir, J. and Chalfie, M. (2010) Annu. Rev. Biophys. 39, 111. 2. Schild, L. (2010) Biochim. Biophys. Acta 1802, 1159. 3. Soundararajan, R. et al. (2010) J. Biol. Chem. 285, 30363. 4. Diakov, A. and Korbmacher, C. (2004) J. Biol. Chem. 279, 38134. 5. Chang, S.S. et al. (1996) Nat. Genet. 12, 248.
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