Sodium Leak Channel Non-Selective Protein (Nalcn) (extracellular) Antibody
Affinity purified polyclonal antibody
|Reactivity||Human, Mouse, Rat|
|Calculated MW||200491 Da|
|Homology||Mouse, human - identical.|
|Other Names||Sodium leak channel non-selective protein, Four domain-type voltage-gated ion channel alpha-1 subunit, Voltage gated channel-like protein 1, Nalcn, Nca, Vgcnl1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)SMMFESPFRRVMH, corresponding to amino acid residues 902-914 of rat Sodium leak channel non-selective protein (Accession Q6Q760). Extracellular, S1-S2 domain III.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The existence of resting K+ conductance has been known for some time. Only recently has the Nalcn (sodium leak channel non-selective protein) has been found responsible for the resting Na+ conductance1. Nalcn is a member of the NaV and CaV channels family which have four homologous repeats of six transmembrane domains. One structural difference is that Nalcn is less positively charged in the voltage sensing segment1. The protein forms voltage-independent non-inactivating channel which is permeable to Na+, K+ and Ca2+ ions1,2. Evidence suggests that Nalcn is a non-redundant and thus essential channel since knock-out mice display disrupted respiratory rhythm and die with 24 hours of birth. In addition, isolated neurons from these mice have decreased excitability1. The channel is extensively expressed in the central nervous system1, heart, pituitary and adrenal glands and pancreatic islet cells2,3. In pancreatic islets, Nalcn is activated by acetylcholine via its physical association with M3 muscarinic receptor, and potentiates insulin secretion induced by glucose uptake2-4.
References 1. Lu, B. et al. (2007) Cell 129, 371. 2. Gilon, P. and Rorsman, P. (2009) EMBO Rep. 10, 963. 3. Swayne, L.A. et al. (2009) EMBO Rep. 10, 873. 4. Swayne, L.A. et al. (2010) Islets 2, 54.
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