|Calculated MW||208867 Da|
|Homology||Mouse- 15/17 amino acid residues identical; human- 14/17 amino acid residues identical.|
|Other Names||Sodium channel protein type 4 subunit alpha, Mu-1, SkM1, Sodium channel protein skeletal muscle subunit alpha, Sodium channel protein type IV subunit alpha, Voltage-gated sodium channel subunit alpha Nav14, Scn4a|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide EDKELKDNHILNHVG(C), corresponding to amino acid residues 877-891 of rat Nav1.4 (Accession P15390 ).ֲ ֲ Intracellular loop between domains II and III.|
|Peptide Confirmation||Confirmed by amino acid analysis and masspectrography.|
|Application Details||Western blot analysis (WB): - Mouse muscle lysate (see Boyer, J.G. et al. (2013) in Product Citations). - Human cardiac cells (see Baroni, D. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Mouse LVA myocytes (1:100) (see Ednie, A.R. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Voltage-gated sodium channels (Nav) are essential for the generation of action potentials and for cell excitability.1 Nav channels are activated in response to depolarization and selectively allow flow of Na+ ions. The Nav channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.2-3 Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. To date, nine Nav α subunits have been cloned and named Nav1.1-Nav1.9.4-5 Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6 The isoform Nav1.4, is primarily expressed in skeletal muscle.7 Different missense mutations in the gene for the Nav1.4 are correlated with several muscular diseases such as, Paramyotonia congenital (PMC), PMC without cold paralysis, potassium-aggravating myotonia, and hyperkalemic periodic paralysis.8
References 1. Wu, L. et al. (2002) NeuroReport 13, 2547. 2. Fang, X. et al. (2002) J. Neurosci. 22, 7425. 3. Fjell, J. et al. (2000) NeuroReport 11, 199. 4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. 5. Lai, J. et al. (2003) Curr.Opin. Neurobiol 13, 291. 6. Isom, L.L. (2001) Neuroscientist 7, 42. 7. Catterall, W.A. et al. (2005) Pharmacological Reviews 57, 397. 8. Sasaki, R. et al. (1999) Arch. Neurol. 56, 692.
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