|Calculated MW||71995 Da|
|Homology||Mouse - identical; human - 14/15 amino acid residues identical.|
|Other Names||Amiloride-sensitive sodium channel subunit beta, Beta-NaCH, Epithelial Na(+) channel subunit beta, Beta-ENaC, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB, Scnn1b|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide EFNYRTIEESPANNI(C), corresponding to amino acid residues 498-513 of rat ENaC־² (Accession ֲ P37090). Extracellular.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The amiloride-sensitive epithelial Na+ channel (ENaC) family includes 4 members: ENaCα, β, γ and δ. The ENaC subunits have a conserved topology consisting of two membrane-spanning domains with intracellular N and C-termini and a large glycosylated extracellular region. The functional ENaC channel is a heteromer with a presumed stoichiometry of α2βγ while the δ subunit can substitute for the α subunit in some tissues. Interestingly, neither the β nor the γ subunits are capable of producing any current when expressed alone in heterologous systems, while in these systems the simultaneous presence of all three ENaC subunits will produce Na+ currents that resemble the endogenous channel. The ENaC channel is located in the luminal (apical) plasma membrane of several epithelial tissues such as kidney, lung, salivary glands and skin where it enables entry of Na+ into the cell along its electrochemical gradient and thus has a central role in the maintenance of renal Na+ balance as well as liquid balance in the lung. The central role of ENaC in the regulation of Na+ homeostasis and hence blood pressure is underscored by the identification of two human diseases that arise from either gain- or loss-of-function mutations of the ENaC channel. Liddle’s syndrome is an inherited form of hypertension that stems from a dominant mutation of the ENaC channel (in either the ß or γ subunits) that results in excessive activity of the channel and hence increased Na+ absorption. Conversely, pseudoaldosteronism type I (PHA) is a dysfunction characterized by hypotension due to poor Na+ absorption that is associated with loss-of-function mutations which may occur in each of the three ENaC subunits. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat ENaCβ. The Anti-ENaCβ antibody (#AG1026) can be used in western blot and immunohistochemistry applications. It has been designed to recognize ENaCβ from rat, human and mouse samples.
References 1. Kellenberger, S. and Schild, S. (2002) Physiol. Rev. 82, 735. 2. Snyder, P.M. (2002) Endocrine Rev. 23, 258. 3. Garty, H. and Palmer, L.G. (1997) Physiol. Rev. 77, 359.
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