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ENaCbeta Antibody

Affinity purified polyclonal antibody

  • WB - ENaCbeta Antibody AG1026-025
    Western blot analysis of rat kidney (lanes 1 and 3) and lung (lanes 2 and 4) lysates:
    1, 2. Anti-EnaCβ antibody (#AG1026), (1:200).
    3, 4. Anti-EnaCβ antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P37090
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 71995 Da
Homology Mouse - identical; human - 14/15 amino acid residues identical.
Additional Information
Gene ID 24767
Other Names Amiloride-sensitive sodium channel subunit beta, Beta-NaCH, Epithelial Na(+) channel subunit beta, Beta-ENaC, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB, Scnn1b
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide EFNYRTIEESPANNI(C), corresponding to amino acid residues 498-513 of rat ENaC־² (Accession ֲ P37090). Extracellular.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Citations (0)

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The amiloride-sensitive epithelial Na+ channel (ENaC) family includes 4 members: ENaCα, β, γ and δ. The ENaC subunits have a conserved topology consisting of two membrane-spanning domains with intracellular N and C-termini and a large glycosylated extracellular region.   The functional ENaC channel is a heteromer with a presumed stoichiometry of α2βγ while the δ subunit can substitute for the α subunit in some tissues. Interestingly, neither the β nor the γ subunits are capable of producing any current when expressed alone in heterologous systems, while in these systems the simultaneous presence of all three ENaC subunits will produce Na+ currents that resemble the endogenous channel.    The ENaC channel is located in the luminal (apical) plasma membrane of several epithelial tissues such as kidney, lung, salivary glands and skin where it enables entry of Na+ into the cell along its electrochemical gradient and thus has a central role in the maintenance of renal Na+ balance as well as liquid balance in the lung. The central role of ENaC in the regulation of Na+ homeostasis and hence blood pressure is underscored by the identification of two human diseases that arise from either gain- or loss-of-function mutations of the ENaC channel. Liddle’s syndrome is an inherited form of hypertension that stems from a dominant mutation of the ENaC channel (in either the ß or γ subunits) that results in excessive activity of the channel and hence increased Na+ absorption. Conversely, pseudoaldosteronism type I (PHA) is a dysfunction characterized by hypotension due to poor Na+ absorption that is associated with loss-of-function mutations which may occur in each of the three ENaC subunits.   Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat ENaCβ. The Anti-ENaCβ antibody (#AG1026) can be used in western blot and immunohistochemistry applications. It has been designed to recognize ENaCβ from rat, human and mouse samples.


References 1. Kellenberger, S. and Schild, S. (2002) Physiol. Rev. 82, 735. 2. Snyder, P.M. (2002) Endocrine Rev. 23, 258. 3. Garty, H. and Palmer, L.G. (1997) Physiol. Rev. 77, 359.

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$ 247.00
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$ 475.00
Cat# AG1026-025
Availability: 2-3 weeks
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