|Calculated MW||59227 Da|
|Homology||Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical.|
|Other Names||Acid-sensing ion channel 3, ASIC3, Amiloride-sensitive cation channel 3, Dorsal root ASIC, DRASIC, Asic3, Accn3, Drasic|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3 (Accession O35240).ֲ ֲ Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Immunohistochemistry (IH): - Rat trigeminal ganglia (1:400) (see Yan, J. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
ASIC3 is a member of a family of Na+ channels that are activated by external protons. The family includes another three members ASIC1, ASIC2 and ASIC4. The ASICs are in fact part of a larger superfamily termed degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N and C termini. The functional channel is composed of 4 subunits that can be assembled as homo or heterotetramers with the other ASIC subunits. A drop in external pH opens the channel resulting in an inward cation current that is quickly inactivated even in the continuous presence of protons in the medium, although a small residual current may persist. Several lines of evidence indicate that ASIC3 may function as a pain sensor. First, it is specifically located in dorsal root ganglia (DRG) neurons and in nociceptors that are involved in pain detection and transmission. In addition, in conditions such as inflammation and cardiac ischemia that include tissue acidosis and thus pain, ASIC3 currents have been detected. Finally, ASIC3 deficient mice display altered sensitivity to high intensity pain produced by heat or acid. Interestingly, while in rats ASIC3 is expressed almost exclusively in DRG neurons, in humans its expression is more widespread suggesting a more extensive role in human nociception.
References 1. Kellenberger, S. and Schild, L. (2002) Physiol. Rev. 82, 735. 2. Chen, C. C. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 8992. 3. Price, M.P. et al. (2001) Neuron 32, 1071.
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