|Calculated MW||201845 Da|
|Homology||Mouse - 15/18 amino acid residues identical; human - 14/18 amino acid residues identical.|
|Other Names||Sodium channel protein type 11 subunit alpha, NaN, Sensory neuron sodium channel 2, Sodium channel protein type XI subunit alpha, Voltage-gated sodium channel subunit alpha Nav19, Scn11a, Nan, Sns2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat Nav1.9 (Accession O88457 ).ֲ ֲ Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Rat sciatic nerve and rat DRG (1:500) (see Su, Y.Y. et al. (2013) in Product Citations). - Rat L4-6 DRG lysates (1:200), (see Yu, Y.Q. et al. (2011) in Product Citations). Immunohistochemistry (IH): - Rat L4-6 DRG sections (1:200), (see Yu, Y.Q. et al. (2011) in Product Citations). - Human trigeminal ganglia and dental pulp (1:100) (see Wells, J.E. et al. (2007) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.9. 2-3 Expression of the α subunit is developmentally and tissue specific. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.4-5 TTX-resistant channels have been suggested to play an important role in nociceptive transmission. Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.9 channel (also called NAN or SNS2) is preferentially expressed in small-diameter DRG neurons.4-5 Recently, it was shown that BDNF activates Nav1.9 channels in the hippocampus suggesting that its expression is not restricted to DRG alone. 6
References 1. Wu, L. et al. (2002) NeuroReport 13, 2547. 2. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. 3. Lai, J. et al (2003) Curr.Opin. Neurobiol 13, 291. 4. Fang, X. et al (2002) J. Neurosci. 22, 7425. 5. Fjell, J. et al (2000) NeuroReport 11, 199. 6. Blum, R. et al (2002) Nature 419, 687.
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