|Homology||Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical.|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat Nav1.8 (Accession Q63554). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Rat sciatic nerve and rat DRG (1:2000) (see Su, Y.Y. et al. (2013) in Product Citations). - Rat colon lysates (see Hu, S. et al. (2013) in Product Citations). - Rat L4-6 DRG lysates (1:200), (see Yu, Y.Q. et al. (2011) in Product Citations). Immunohistochemistry (IH): - Rat DRG (1:1000) (see Su, Y.Y. et al. (2013) in Product Citations). - Rat L4-6 DRG sections (1:200), (see Yu, Y.Q. et al. (2011) in Product Citations). - Rat L4-6 DRG sections (1:500), (see Belkouch, M. et al. (2011) in Product Citations). - Human Neuromas (1:100) (see Black, J.A. et al. (2008) in Product Citations). Immunocytochemistry (IC): - ND7-23 cells (1:1000) (see Su, Y.Y. et al. (2013) in Product Citations). - Rat cultured DRGs (1:500), (see Belkouch, M. et al. (2011) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.9 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.9. 2-3 The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.4-5 Expression of the α subunit isoform is developmentally and tissue specific. Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.8 channel (also called SNS, SCN10A and PN3) is mainly expressed in small-diameter DRG neurons.4-6 TTX-resistant channels have been suggested to play an important role in nociceptive transmission. Recently, involvement of NaV1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both, mRNA and protein, in Purkinje cells of MS patients and also in animal models.6
References 1. Wu, L. et al. (2002) NeuroReport 13, 2547. 2. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. 3. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291. 4. Fang, X. et al. (2002) J. Neurosci. 22, 7425. 5. Fjell, J. et al. (2000) NeuroReport 11, 199. 6. Renganathan, M. et al. (2003) Brain Res. 959, 235.
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