|Homology||Mouse, human - identical.|
|Other Names||ACCN4; Acid-sensing ion channel 4; amiloride-sensitive cation channel 4|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CKIKFAEEDAKPKEKEAGDE, corresponding to amino acid residues 7-26 of rat ASIC4 (Accession Q9QYV9).ֲ ֲ Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Acid-sensing ion channels (ASICs) are Na+ channels activated by external protons. ASIC4 is a member of the ASIC family that includes another three members: ASIC1, ASIC2 and ASIC3. The ASIC family is in fact part of a large channel superfamily known as degenerin/epithelial Na+ channels (DEG/ENaC) and share the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N and C termini. The functional ASIC channel is composed of 4 subunits, which can form either a homo or heterotetramer. The subunit composition of the tetrameric channel will determine its biophysical properties such as pH sensitivity, inactivation kinetics and channel pharmacology. In fact, the ASIC4 protein does not appear to function as a proton-gated channel when expressed alone in heterologous systems and therefore a modulatory role for this subunit has been proposed. Expression of the ASIC4 protein has been detected in brain, pituitary gland, spinal cord and inner ear. The functional significance of ASIC4 expression in these tissues still awaits clarification. Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of rat ASIC4. The Anti-ASIC4 antibody (#AG1030) can be used in western blot analysis. It has been designed to recognize ASIC4 from rat, human and mouse samples.
References 1. Grunder, S. et al. (2000) Neuroreport 11, 1607. 2. Akopian, A.N. et al. (2000) Neuroreport 11, 2217. 3. Kellenberger, S. et al. (2002) Physiol. Rev. 82, 735.
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