|Calculated MW||57709 Da|
|Homology||Rat, mouse - identical.|
|Other Names||Acid-sensing ion channel 2, ASIC2, Amiloride-sensitive brain sodium channel, Amiloride-sensitive cation channel 1, neuronal, Amiloride-sensitive cation channel neuronal 1, Brain sodium channel 1, BNC1, BNaC1, Mammalian degenerin homolog, ASIC2, ACCN, ACCN1, BNAC1, MDEG|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide DLKESPSEGSLQPSSIQC, corresponding to amino acid residues 2-18 of human ASIC2a (Accession # Q16515). Intracellular, N-terminus.The antibody is specific for ASIC2a and will not recognize ASIC2b.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.3 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
ASIC2a is a member of a family of Na+ channels that are activated by external protons. The family includes another three members ASIC1, ASIC2 and ASIC4. The ASICs are in fact part of a larger superfamily termed degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N and C termini. There are two recognized splice variants of the ASIC2 gene that differ on their N-termini, ASIC2a and ASIC2b that have different tissue distributions and functions. ASIC2a is highly expressed in the central nervous system (CNS) and is less expressed in the peripheral nervous system (PNS) while ASIC2b is prominently expressed in the latter. The functional channel is composed of 4 subunits that can be assembled as homo or heterotetramers with the other ASIC subunits. The ASIC2b splice variant does not appear to be functional when expressed alone but it can modify the properties of ASIC2a and ASIC3 when co-expressed. The ASIC2 protein has been proposed to be involved in mechanosensation and sensory transduction. Abgent is pleased to offer a highly specific antibody directed against a region situated at the N-terminus of human ASIC2a. The Anti-ASIC2a antibody (#AG1032) can be used in western blot analysis. It has been designed to recognize human, rat and mouse ASIC2a samples.The antibody is specific for ASIC2a and will not recognize the ASIC2b variant.
References 1. Kellenberger, S. et al. (2002) Physiol. Rev. 82, 735. 2. Krishtal, O. et al. (2003) Trends Neurosci. 26, 477. 3. Price, M.P. et al. (2000) Nature 407, 1007.
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