|Calculated MW||74066 Da|
|Homology||Human - 13/17 amino acid residues identical.|
|Other Names||Amiloride-sensitive sodium channel subunit gamma, Epithelial Na(+) channel subunit gamma, Gamma-ENaC, Gamma-NaCH, Nonvoltage-gated sodium channel 1 subunit gamma, SCNEG, Scnn1g|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)YGVKESRKRREAGS, corresponding to amino acid residues 129-142 of rat ENaC־³ֲ (Accession P37091). Extracellular.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The amiloride-sensitive epithelial Na+ channel (ENaCs) family includes 4 members: ENaCα, β, γ and δ. The ENaC channel is located in the luminal (apical) plasma membrane of several epithelial tissues such as kidney, lung, salivary glands and skin. The functional channel is believed to be a multimer including a α (or δ) and a β and γ subunits with a likely stoichiometry of α2βγ. ENaC channel enable entry of Na+ into the cell along its electrochemical gradient and thus has a central role in the maintenance of renal Na+ balance (and hence blood pressure) and liquid balance in the lung. Indeed, genetic mutations in the ENaC subunits causes Liddle’s syndrome (a form of hypertension) or pseudohypoaldosteronism type 1 (PHA) that is characterized by hypotension. The ENaC channel is voltage-independent and is constitutively active in epithelia although it is modulated by several different mechanisms. One of the main mechanisms is the controlled internalization of the channel that is dependent on the β or γ subunits. Indeed, mutations in the C-termini of these subunits reduce endocytosis of the channel leading to the accumulation of ENaC in the cell membrane and causing a phenotype that is consistent with that of Liddle’s syndrome. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat ENaCγ. The Anti-ENaCγ antibody (#AG1033) can be used in western blot analysis. It has been designed to recognize ENaCγ from rat and human samples.
References 1. Kellenberger, S. et al. (2003) J. Biol.Chem. 278, 29153. 2. Snyder, P.M. (2002) Endocrine Rev. 23, 258.
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