|Reactivity||Human, Mouse, Rat|
|Calculated MW||226039 Da|
|Homology||Rabbit - 14/15 amino acid residues identical; human, mouse - 13/15 amino acid residues identical.|
|Other Names||Sodium channel protein type 9 subunit alpha, Peripheral sodium channel 1, PN1, Sodium channel protein type IX subunit alpha, Voltage-gated sodium channel subunit alpha Nav17, Scn9a|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat Nav1.7ֲ (Accession O08562).ֲ ֲ Intracellular loop between domains I and II.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Rat colon lysate (see Wang, Y. et al. (2012) in Product Citations). Immunohistochemistry (IH): - Human cervical tissue (1:25) (see Hernandez-Plata, E. et al. (2012) in Product Citations). - Mouse dorsal root ganglia (DRGs) (1:1000) (see Amaya, F. et al. (2006) in Product Citations). Immunocytochemistry (IC): - Human cervical cancer cell line (1:25) (see Hernandez-Plata, E. et al. (2012) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 Nav channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine Nav α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3 Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. Expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6-8 NaV1.7 is predominantly expressed in dorsal root ganglions (DRG) of the peripheral nervous system. Dominant gain of function mutations in the NaV1.7 gene are associated with erythermalgia (a rare autosomal disease characterized by sporadic burning pain accompanied by redness and heat in the extremities).9-11 Loss, or function mutations in NaV1.7 channels, leads to complete ablation of pain perception in humans.11 These recent findings highlight the role of this NaV isoform and the subset of DRG neurons that express this channel in physiological pain sensation.
References 1. Wu, L. et al. (2002) NeuroReport 13, 2547. 2. Fang, X. et al. (2002) J. Neurosci. 22, 7425. 3. Fjell, J. et al. (2000) NeuroReport 11, 199. 4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. 5. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291. 6. Isom, L.L. (2001) Neuroscientist 7, 42. 7. Catterall, W.A. et al. (2003) Pharmacol Rev 55, 575. 8. Catterall, W.A. et al. (2005) Pharmacol. Rev. 57, 397. 9. Yang, Y. et al. (2004) J. Med. Genet. 41, 171. 10. Cummins, T.R. et al. (2004) J. Neurosci. 24, 8232. 11. Dray, A. (2008) Br. J. Anaesth. 101, 48.
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