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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   NaV1.5 Antibody   

NaV1.5 Antibody

Affinity purified polyclonal antibody

  • WB - NaV1.5 Antibody AG1035-025
    Western blot analysis of rat heart membranes: 1. Anti-Nav1.5 antibody (#AG1035), (1:200).   
    2. Anti-Nav1.5 antibody, preincubated with the control peptide antigen. 
  • WB - NaV1.5 Antibody AG1035-025
    Western blot analysis of Nav1.5 in human-Nav1.5 transfected HEK-293 cells: 1. Anti-Nav1.5 antibody (#AG1035), (1:200).   
    2. Anti-Nav1.5 antibody, preincubated with the control peptide antigen.
  • IHC - NaV1.5 Antibody AG1035-025
    Expression of Nav1.5 in rat heart Immunohistochemical staining of Nav1.5 in rat myocardium paraffin-embedded sections using Anti-Nav1.5 antibody (#AG1035), (1:100). Staining is specific for cardiomyocytes while smooth muscles cells in the small vessels are negative (red arrows). Hematoxilin is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15389
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 227367 Da
Homology Mouse- identical; human - 17/19 amino acid residues identical.
Additional Information
Gene ID 25665
Other Names Sodium channel protein type 5 subunit alpha, Sodium channel protein cardiac muscle subunit alpha, Sodium channel protein type V subunit alpha, Voltage-gated sodium channel subunit alpha Nav15, Scn5a
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide DRLPKSDSEDGPRALNQLS(C), corresponding to amino acid residues 493-511 ofֲ rat NaV1.5 (accession number P15389). Intracellular loop between domains I and II.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Western blot analysis (WB): - Mouse muscle lysate (see Boyer, J.G. et al. (2013) in Product Citations). - Mouse heart lysate (1:200) (see Watanabe, H. et al. (2011) in Product Citations).   Immunoprecipitation (IP): - Mouse cardiac myocytes (1:500) (see Malhotra, J.D. et al. (2001) in Product Citations).   Immunocytochemistry (IC): - Neonatal rat ventricular cardiomyocytes (NRCs) (1:200) (see Casini, S. et al. (2010) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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Voltage-gated Na+ channels (NaV) are responsible for myocardial conduction and maintenance of the cardiac rhythm and are essential for the generation of action potentials and cell excitability.1   Dysfunction or disregulation of cardiac sodium channels can cause several disorders, including cardiac arrhythmias.   The majority of Na+ channels in the mammalian heart are Tetrodotoxin (TTX)-insensitive NaV1.5.2  The putative structure of NaV1.5 consists of four homologous domains (I-IV), each containing six transmembrane segments (S1-S6). Mutations in the C-terminus of NaV1.5 were described in connection to Long QT syndrome and Brugada syndrome.1-2 Recent data have demonstrated selective expression of NaV1.5 in the mouse central nervous system and implicated a role for NaV1.5 in the physiology of the central nervous system.1


References 1. Wu, L. et al. (2002) NeuroReport 13, 2547. 2. Liu, C.J. et al. (2003) J. Biol. Chem. 278, 1029.  

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$ 375.00
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$ 575.00
Cat# AG1035-025
(40 western blots)
Availability: 2-3 weeks
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