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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   NaV1.2 Antibody   

NaV1.2 Antibody

Affinity purified polyclonal antibody

     
  • WB - NaV1.2 Antibody AG1037-025
    Western blot analysis of rat brain membranes:
    1. Anti-Nav1.2 antibody (#AG1037), (1:200).
    2. Anti-Nav1.2 antibody, preincubated with the control peptide antigen.
  • WB - NaV1.2 Antibody AG1037-025
    Immunoprecipitation of rat brain lysate: 1. Rat brain lysates
    2. Lysates immunoprecipitated with Anti-Nav1.2 antibody (#AG1037), (6 µg).
    3. Lysates immunoprecipitated with pre-immune rabbit serum.
    The lower arrow indicates the IgG heavy chain.
    Western blot analysis was performed with Anti-Nav1.2 antibody.
  • IHC - NaV1.2 Antibody AG1037-025
    Expression of Nav1.2 in mouse hippocampus Immunohistochemical staining of mouse hippocampus using Anti-Nav1.2 antibody (#AG1037 ). A. NaV1.2 (red) is present in dendrites of pyramidal neurons in the CA3 region. B. Staining of interneurons in the pyramidal layer with mouse anti-Parvalbumin (green) demonstrates the restriction of NaV1.2 to dendrites (arrows) extending from the pyramidal layer (P). C. Confocal merge of panels A and B.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IP
Primary Accession P04775
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 227874 Da
Homology Human - identical. NaV1.3 - 12/15 amino acid residues identical.
Additional Information
Gene ID 24766
Other Names Sodium channel protein type 2 subunit alpha, Sodium channel protein brain II subunit alpha, Sodium channel protein type II subunit alpha, Voltage-gated sodium channel subunit alpha Nav12, Scn2a, Scn2a1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat Nav1.2 (Accession P04775).ֲ ֲ Intracellular loop between domains I and II .
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Western blot analysis (WB): - Human and Mouse brain (see Verret, L. et al. (2012) in Product Citations). - Mouse heart (see Haufe, V. et al. (2005) in Product Citations). - Human brain (see Kamiya, K. et al. (2004) in Product Citations). - Rat brain (see Malhotra, J.D. et al. (2001) in Product Citations). - Mouse brain (see Planells-Cases, R. et al. (2000) in Product Citations).   Immunoprecipitation (IP): - Mouse heart (see Haufe, V. et al. (2005) in Product Citations).   Immunocytochemistry (IC): - Mouse heart (see Haufe, V. et al. (2005) in Product Citations). - Human brain (see Kamiya, K. et al. (2004) in Product Citations). - Mouse heart (see Lei, M. et al. (2004) in Product Citations). - Mouse brain (see Planells-Cases, R. et al. (2000) in Product Citations). - Mouse brain (see Zhou, D. et al. in Product Citations).   Immunohistochemistry (IH): - Rat brain sections (1:250) (see Hargus, N.J. et al. (2013) in Product Citations). - Rat brain (see Hu, W. Et al. (2009) in Product Citations). - Mouse heart (see Lei, M. et al. (2004) in Product Citations). - Guinea pig intestine (see Hanani, M. et al. (2000) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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Background

Voltage-gated sodium channels (Nav) are essential for the generation of action potentials and for cell excitability.1 Nav channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine Nav α subunits have been cloned and named Nav1.1-Nav1.9.4-5  The Nav channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.6 and Nav1.7)  and TTX-resistant (Nav1.5, Nav1.8 and Nav1.9).2-3 Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7   Nav1.2 is primarily expressed in the central nervous system (CNS) and is localized in unmyelinated or premyelinated axons and dendrites. Mutations in the Nav1.2 channel have been identified in different types of epilepsy.

References

References 1. Wu, L. et al. (2002) NeuroReport 13, 2547. 2. Fang, X. et al. (2002) J. Neurosci. 22, 7425. 3. Fjell, J. et al. (2000) NeuroReport 11, 199. 4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. 5. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291. 6. Isom, L.L. (2001) Neuroscientist  7, 42. 7. Catterall, W.A. et al. (2003) Pharmacol Rev  55, 579. 8. Catterall, W.A. et al. (2008) J. Neurosci. 28, 11768.

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