|Calculated MW||552042 Da|
|Homology||Rat, mouse - identical.|
|Other Names||Ryanodine receptor 3, RYR-3, RyR3, Brain ryanodine receptor-calcium release channel, Brain-type ryanodine receptor, Type 3 ryanodine receptor, RYR3, HBRR|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CHIAHVNPAAEEDLK, corresponding to amino acid residues 935-949 of human Ryanodine Receptor 3 (Accession Q15413). Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The primary intracellular Ca2+ storage/release organelles in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells. The ER and SR contain two Ca2+ release channel families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs).3 The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3).3 The Ryanodine receptors are homotetrameric proteins generating a Ca2+ conducting channel. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine (poisonous alkaloid found in the South American plant Ryania speciosa to the Ryanodine receptor causes two major changes in the channel: a reduction in single-channel conductance and a marked increase in the open state probability, leading to an overall increase/decrease in the Ca2+ release capability of the channel. RyR3 is the most widely expressed RyR channel which is predominantly expressed in smooth muscle tissues and certain regions of the brain. Abgent is pleased to offer a highly specific antibody directed against an epitope putatively located at the cytoplasmic region of the human Ryanodine receptor 3 (RyR3). The Anti-Ryanodine Receptor 3 antibody (#AG1038) can be used in western blot analysis and Immunohistochemical applications. It has been designed to recognize RyR3 from human, rat and mouse samples.
References 1. Chakrabarti, R. And Chakrabarti, R. (2006) J.Cell. Biochem. 99, 1503. 2. Eisner, D.A. et al. (2005) Exp.Physiol. 90, 3. 3. Hamilton, S.L. (2005) Cell Calcium 38, 253 4. Fill, M. and Copello, J.A. (2002) Physiol Rev. 82, 893.
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